Claim for cosmetics

H295R Steroidogenesis Assay – OECD 456

Regulatory reference: According to OECD 456.
Claim: No endocrin disruption
Application field: Raw ingredients and finished products.
Description of the test: The purpose of the test is identifying substances that interfere with 17-ß estradiol and testosterone production.
Posted in Cosmetic raw materials, No endocrin disruption, Raw materials for medical devices, Claim for cosmetics, Claim for medical devices

Hyaluronic acid synthesis test

Regulatory reference: Proprietary method.
Claim: Anti-aging and Reduces signs of aging
Application field: Raw materials and finished products.
Description of the test: In vitro evaluation of the synthesis of hyaluronic acid up to 48h following exposure to the sample, in several concentrations, on skin and dermal papillae fibroblasts.
Posted in Anti-aging, Anti age, Reduces signs of aging, Claim for cosmetics

In vitro test for the evaluation of the protective effect against air pollution

Regulatory reference: Proprietary method
Claim: Antioxidant and Anti-pollution
Application field: Raw materials and finished products.
Description of the test: In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model.
Posted in Anti age, Sunscreens, Claim for OTC, Antioxidant, Anti-pollution, Cosmetic raw materials, Make up, Moisturizers, Claim for cosmetics

Cytotoxicity test for the identification of substances with acute oral toxicity LD50>2000 mg/kg

Regulatory reference: OECD 129: on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests;
EURL-ECVAM 2011: follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system.
Claim: Biocompatible and Non citotoxic
Application field: Chemical substances or mixtures.
Description of the test: The in vitro LD50 prediction test is designed to test chemicals or mixtures by evaluating their cytotoxic effect on an in vitro model of BALB/c 3T3 murine fibroblasts.
Posted in Non citotoxic, Cosmetic raw materials, Claim for cosmetics, Biocompatible

Evaluation of the enzymatic inhibition of Tyrosinase

Regulatory reference: Proprietary method
Claim: Depigmenting action
Application field: Active ingredients, mixtures or finished products.
Description of the test: Evaluation of the efficacy of a sample in inhibiting the activity of the tyrosinase enzyme in a controlled in vitro direct reaction.
Posted in Blemish reduction, depigmenting, tanning, Depigmenting action, Claim for cosmetics

Evaluation of proliferation and protein synthesis on dermal papilla mesenchymal cells

Regulatory reference: Proprietary method
Claim: Anti-hair loss and Strenghtening
Application field: Active ingredients and finished products.
Description of the test: Quantitative evaluation of the effects of a finished product or active ingredient on cell proliferation and protein synthesis through MTT test and total protein quantification after different periods of exposure.
Posted in Anti-hair loss, Strenghtening, Hair products, Claim for cosmetics

Ocular irritation test on reconstructed corneal epithelium

Regulatory reference: OECD n° 492
Claim: Non-irritant for the eyes
Application field: Chemical substances or mixtures.
Description of the test: Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action.
Posted in Cosmetic raw materials, Detergents, Make up, Claim for cosmetics, Non-irritant for the eyes

Clinical simulation of use and ophthalmological evaluation on 20 healthy volunteers

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Ophthalmologically tested
Application field: Eye contour cosmetics or products that require to confirm the compatibility with the periocular mucosa in normal usage conditions.
Description of the test: An ophthalmology specialist will inspect the target area, and especially the eyelids, before the application using a fissure lamp, in order to confirm the absence of swelling, hyperemia, dryness or desquamation. The inspection also includes any alterations of the eyelashes.
In the eye itself, the integrity of the cornea and conjunctiva, the absence of hyperemia, and signs of conjunctival or tarsal irritation are verified. Finally, the absence of qualitative and quantitative alterations in the tear film.
The specialist then evaluates and scores erythemal response, tearing, edema and dryness after the product has been used for 30 minutes, 2 days and 30 days to quantify possible alterations induced by the sample.
The volunteers also provide subjective evaluations of itching, burning, stinging or foreign body response feeling, and/or sight clouding, at the same endpoints, using the VAS (Visual Analogical Scale) score system.
Posted in Make up, Claim for cosmetics, Ophthalmologically tested

Evaluation of hair restructuring efficacy

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Anti-hair loss
Application field: Hair products and cosmetics products for scalp treatment.
Description of the test: In vivo test: performed on selected volunteers with specific trichological features (dry and damaged hair). Volunteers will apply the product according to pre-determined frequency and methods. 10 hairs from each subject will be gathered at T=0 and T= X days (duration to be defined with the customer). Dynamometric measurements will be performed in order to evaluate the increase in tensile strength of the hairs and their resistance to breakage. Subjective evaluations will be provided by volunteers using an open and multiple choices questionnaire to verify the perceived efficacy and other parameters such as effects on hair volume and agreeability.
In vitro test: standardized locks of human hair (several types are available according to preferences) will be treated with a damaging mixture in controlled conditions. Part of the locks will then undergo treatment with the product. Comparative imaging will be performed under tangent light to highlight improvement of hair brightness. 2 samples will be taken from each lock to evaluate improvement by phase contrast microscopy or SEM.
Posted in Anti-hair loss, Hair products, Claim for cosmetics

Sebum-regulation activity test and count of closed and open comedones

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Non-comedogenic and Sebum-regulator
Application field: Visage and body cosmetics for oily skin.
Description of the test: Sebometric measurements in triplicate performed with Sebometer® at T=0 and T=X days after the treatment. Measurements will be performed on the cheeks after treatment. The untreated forehead will be used as a control. The measurements will compensate for circadian rhythms and will therefore be performed at the same time during the day. Open and closed comedones will be counted under Wood’s lamp illumination. Clinical evaluation of high resolution imaging will be performed using a contrast system at T=0 and after X days of treatment.
Posted in Acne, Sebum-regulator, Non-comedogenic, Claim for cosmetics