Cosmetic raw materials

H295R Steroidogenesis Assay – OECD 456

Regulatory reference: According to OECD 456.
Claim: No endocrin disruption
Application field: Raw ingredients and finished products.
Description of the test: The purpose of the test is identifying substances that interfere with 17-ß estradiol and testosterone production.
Posted in Cosmetic raw materials, No endocrin disruption, Raw materials for medical devices, Claim for cosmetics, Claim for medical devices

In vitro test for the evaluation of the protective effect against air pollution

Regulatory reference: Proprietary method
Claim: Antioxidant and Anti-pollution
Application field: Raw materials and finished products.
Description of the test: In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model.
Posted in Anti age, Sunscreens, Claim for OTC, Antioxidant, Anti-pollution, Cosmetic raw materials, Make up, Moisturizers, Claim for cosmetics

Cytotoxicity test for the identification of substances with acute oral toxicity LD50>2000 mg/kg

Regulatory reference: OECD 129: on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests;
EURL-ECVAM 2011: follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system.
Claim: Biocompatible and Non citotoxic
Application field: Chemical substances or mixtures.
Description of the test: The in vitro LD50 prediction test is designed to test chemicals or mixtures by evaluating their cytotoxic effect on an in vitro model of BALB/c 3T3 murine fibroblasts.
Posted in Non citotoxic, Cosmetic raw materials, Claim for cosmetics, Biocompatible

Ocular irritation test on reconstructed corneal epithelium

Regulatory reference: OECD n° 492
Claim: Non-irritant for the eyes
Application field: Chemical substances or mixtures.
Description of the test: Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action.
Posted in Cosmetic raw materials, Detergents, Make up, Claim for cosmetics, Non-irritant for the eyes

In vitro short term exposure test

Regulatory reference: OECD n° 491
Claim: Non-irritant for the eyes (CLP Labelling)
Application field: Raw ingredients or finished products.
Description of the test: Short term exposure analysis by application of each tested substance on SIRC (Statens Seruminstitut Rabbit Cornea) cells for the determination of the effect on cell viability through MTT test.
Posted in Cosmetic raw materials, Detergents, Non-irritant for the eyes (CLP labelling), Make up, Moisturizers, Claim for cosmetics, Non-irritant for the eyes (CLP Labelling)

Skin irritation test on 3D reconstructed epidermis

Regulatory reference: OECD n° 439
Claim: Non-irritant for the skin
Application field: Chemical substances or mixtures.
Description of the test: Evaluation of the irritation potential of chemical substances or mixtures through evaluation of their cytotoxic effect on an in vitro reconstructed model of human skin.
Posted in Cosmetic raw materials, Detergents, Make up, Moisturizers, Claim for cosmetics, Non-irritant for the skin

Antimicrobial activity test vs Propionibacterium acnes

Regulatory reference: Proprietary method
Claim: Anti-acne
Application field: Cosmetics and raw cosmetic ingredients.
Description of the test: Evaluation of the anti-acne efficacy by testing the inhibition of the proliferation of Propionibacterium acnes by an ingredient or finished product.
Posted in Acne, Anti-acne, Cosmetic raw materials

Quantification of trace substances and impurities

Regulatory reference: UE Regulation
Claim: Titer of substances
Application field: Cosmetics and medical devices.
Description of the test: Analysis of trace substances, residues and impurities for quality control purposes through UPLC or GC-MS. Includes sample preparation.
Posted in Fabrics, plastics and polymer patches, Cosmetic raw materials, Raw materials for medical devices, Surgical devices and medications, Claim for cosmetics, Claim for medical devices, Titer of substances

Quantification of the titer of substances

Regulatory reference: Regulation 1223/209
Claim: Titer of substances and Limit of preservatives
Application field: Preservatives, dyes, sunscreen filters.
Description of the test: Quantification method for the measurement of the titer of substances by HPLC, UPLC, GC-MS after preparative steps.
Posted in Heating and cooling products, Mucoadhesive products, Cosmetic raw materials, Detergents, Raw materials for medical devices, Substance-based, Surgical devices and medications, Claim for cosmetics, Claim for medical devices, Titer of substances, Limit of preservatives

OECD 442D in vitro sensitization test

Regulatory reference: OECD 442D
Claim: No-skin sensitizer and Hypoallergenic
Application field: Substances or mixtures.
Description of the test: The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product.
Posted in Cosmetic raw materials, Hypoallergenic, No-skin sensitizer, Claim for cosmetics