Moisturizers

In vitro test for the evaluation of the protective effect against air pollution

Regulatory reference: Proprietary method
Claim: Antioxidant and Anti-pollution
Application field: Raw materials and finished products.
Description of the test: In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model.
Posted in Anti age, Sunscreens, Claim for OTC, Antioxidant, Anti-pollution, Cosmetic raw materials, Make up, Moisturizers, Claim for cosmetics

Moisturizing and deep hydration test with TEWL measurement

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Hydrates the skin
Application field: Cosmetic products.
Description of the test: Test on healthy volunteers with the following instrumental measurements:
  • Corneometric measurements of the epidermal conductivity and TEWL (Trans Epidermal Water Loss) at T=0 and T=2 hours after application;
  • Measurement of the hydration levels at T=0 and T=6 hours from application at a depth of 0.5–1 mm.

Employed instruments:

  • Corneometer® CM285 (Courage-Khazaka GmbH, Germany) – Multiprobe Adapter Systems MPA® (Courage-Kkazaka).
  • Tewameter® TM300 (Courage-Khazaka GmbH, Germany- Multiprobe Adapter Systems MPA® (Courage-Kkazaka).
  • Moisture Meter EpiD – Delphin.
Posted in Hydrates the skin, Moisturizers, Claim for cosmetics

In vitro moisturization test through aquaporin expression

Regulatory reference: Proprietary method
Claim: Hydrates the skin
Application field: Complete products.
Description of the test: Analysis of the expression of aquaporing through real time PCR.
Posted in Hydrates the skin, Moisturizers, Claim for cosmetics

In vitro short term exposure test

Regulatory reference: OECD n° 491
Claim: Non-irritant for the eyes (CLP Labelling)
Application field: Raw ingredients or finished products.
Description of the test: Short term exposure analysis by application of each tested substance on SIRC (Statens Seruminstitut Rabbit Cornea) cells for the determination of the effect on cell viability through MTT test.
Posted in Cosmetic raw materials, Detergents, Non-irritant for the eyes (CLP labelling), Make up, Moisturizers, Claim for cosmetics, Non-irritant for the eyes (CLP Labelling)

Skin irritation test on 3D reconstructed epidermis

Regulatory reference: OECD n° 439
Claim: Non-irritant for the skin
Application field: Chemical substances or mixtures.
Description of the test: Evaluation of the irritation potential of chemical substances or mixtures through evaluation of their cytotoxic effect on an in vitro reconstructed model of human skin.
Posted in Cosmetic raw materials, Detergents, Make up, Moisturizers, Claim for cosmetics, Non-irritant for the skin

In vitro moisturizing efficacy test

Regulatory reference: Proprietary method
Claim: Hydrates the skin
Application field: Finished products.
Description of the test: Evaluation of the moisturizing efficacy of chemical substances or mixtures.
Posted in Hydrates the skin, Moisturizers, Claim for cosmetics

Challenge Test

Regulatory reference: ISO 11930 for cosmetics; European Pharmacopoeia for medical devices
Claim: Efficacy of preservatives
Application field: Cosmetics and medical devices.
Description of the test: Challenge test for the evaluation of the efficacy of preservative systems according to ISO guidelines (for cosmetics) and European Pharmacopoeia (for medical devices).
Posted in Condom and vaginal products, Fabrics, plastics and polymer patches, Heating and cooling products, Mucoadhesive products, Protection and barrier effect, Detergents, Raw materials for medical devices, Substance-based, Surgical devices and medications, Make up, Moisturizers, Claim for cosmetics, Preservative systems, Claim for medical devices, Efficacy of preservatives