Hypoallergenic

OECD 442D in vitro sensitization test

Regulatory reference: OECD 442D
Claim: No-skin sensitizer and Hypoallergenic
Application field: Substances or mixtures.
Description of the test: The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product.
Posted in Cosmetic raw materials, Hypoallergenic, No-skin sensitizer, Claim for cosmetics

OECD 442E in vitro sensitization test

Regulatory reference: OECD 442E
Claim: No-skin sensitizer and Hypoallergenic
Application field: Substances or mixtures.
Description of the test: H-CLAT cytometry method to evaluate possible changes in the expression of cell surface markers (CD86 e CD54) in cultures of THP-1 human monocytes to exclude the related sensitization pathway.
Posted in Cosmetic raw materials, Hypoallergenic, No-skin sensitizer, Claim for cosmetics

DPRA: Direct Peptide Reactivity Assay test

Regulatory reference: OECD 442C
Claim: No-skin sensitizer and Hypoallergenic
Application field: Substances or mixtures without metal compounds.
Description of the test: The DPRA methods chemically quantifies (by LC-MS/MS or HPLC-UV) the covalent bonds of electrophilic chemical substances (oxidants, electron acceptors) to nucleophilic substances, as known to occur between allergens and skin proteins, in order to exclude the product’s activity in the related sensitization pathway.
Posted in Cosmetic raw materials, Hypoallergenic, No-skin sensitizer, Claim for cosmetics