| Regulatory reference: | OECD 442C |
| Claim: | No-skin sensitizer and Hypoallergenic |
| Application field: | Substances or mixtures without metal compounds. |
| Description of the test: | The DPRA methods chemically quantifies (by LC-MS/MS or HPLC-UV) the covalent bonds of electrophilic chemical substances (oxidants, electron acceptors) to nucleophilic substances, as known to occur between allergens and skin proteins, in order to exclude the product’s activity in the related sensitization pathway. |
| Regulatory reference: | Proprietary method |
| Claim: | Facilitates combing |
| Application field: | Hair products. |
| Description of the test: | Dynamometric analysis of the resistance to combing on standardized hair locks. Comparison between treated and untreated wet locks. Results in terms of combing friction (in Newtons) and percentage of friction reduction compared to the untreated control. |
| Regulatory reference: | Proprietary method |
| Claim: | Strenghtening |
| Application field: | Strengthening hair products. |
| Description of the test: | Dynamometric analysis of the tensile strength and resistance to breakage on treated and untreated hairs collected ex vivo from 20 volunteer. Results in terms of tensile strength (in Newtons) and percentage of strengthening compared to the untreated control. |
| Regulatory reference: | Proprietary method |
| Claim: | Sebum-regulator |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the changes in the gene expression of 5α-reductase in human dermal papilla cells. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II. |
| Regulatory reference: | Proprietary method |
| Claim: | Sebum-regulator and Anti-hair loss |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the changes in the gene expression of 5α-reductase in murine Balb/3T3 fibroblasts. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II. |
| Regulatory reference: | Proprietary method |
| Claim: | Sebum-regulator and Anti-hair loss |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Analysis of the in vitro expression of the 5α-reductase enzyme by real time PCR in comparison with untreated substrates for the evaluation of inhibition effects. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II. |
| Regulatory reference: | Proprietary method |
| Claim: | Skin whitening and Depigmenting action |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Evaluation of the changes in the expression of the tyrosinase gene in human melanoma cells. |
| Regulatory reference: | Proprietary method |
| Claim: | Tanning |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the increase of melanin synthesis in human melanoma cells treated with the sample substance or product in comparison with untreated controls. The same test can be used to quantify a reduction in melanin synthesis. |
| Regulatory reference: | Proprietary method |
| Claim: | Anti-cellulite and Sebum-regulator |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Analysis of the activation of the activity of enzymatic lipase in a controlled triglyceride hydrolysis reaction. |
| Regulatory reference: | Proprietary method |
| Claim: | Anti-cellulite |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Evaluation of the reduction of intracellular lipid accumulation in adipocyte cells treated with several concentrations of the tested product, in comparison to untreated adipocytes. |