Publications

Regulatory reference:	OECD 442E
Claim:	No-skin sensitizer and Hypoallergenic
Application field:	Substances or mixtures.
Description of the test:	H-CLAT cytometry method to evaluate possible changes in the expression of cell surface markers (CD86 e CD54) in cultures of THP-1 human monocytes to exclude the related sensitization pathway.

Regulatory reference:	OECD 471
Claim:	Non mutagenic
Application field:	Raw ingredients for medical devices or cosmetics.
Description of the test:	Analysis of genotoxicity of a substance according to OECD 471.

Regulatory reference:	OECD 442C
Claim:	No-skin sensitizer and Hypoallergenic
Application field:	Substances or mixtures without metal compounds.
Description of the test:	The DPRA methods chemically quantifies (by LC-MS/MS or HPLC-UV) the covalent bonds of electrophilic chemical substances (oxidants, electron acceptors) to nucleophilic substances, as known to occur between allergens and skin proteins, in order to exclude the product’s activity in the related sensitization pathway.

Regulatory reference:	Proprietary method
Claim:	Facilitates combing
Application field:	Hair products.
Description of the test:	Dynamometric analysis of the resistance to combing on standardized hair locks. Comparison between treated and untreated wet locks. Results in terms of combing friction (in Newtons) and percentage of friction reduction compared to the untreated control.

Regulatory reference:	Proprietary method
Claim:	Strenghtening
Application field:	Strengthening hair products.
Description of the test:	Dynamometric analysis of the tensile strength and resistance to breakage on treated and untreated hairs collected ex vivo from 20 volunteer. Results in terms of tensile strength (in Newtons) and percentage of strengthening compared to the untreated control.

Regulatory reference:	Proprietary method based on ISO 24443
Claim:	Protects against the high energy visible (HEV) spectrum or Blue light protection
Application field:	Suncreen products.
Description of the test:	Analysis of the absorbance spectrum of a sunscreen product in the HEV spectrum versus blank. Results in terms of absorbance curve and percentage of light blocked in the 400-500 nm specturm.

Regulatory reference:	Proprietary method
Claim:	Sebum-regulator
Application field:	Active ingredients or finished products.
Description of the test:	Quantification of the changes in the gene expression of 5α-reductase in human dermal papilla cells. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II.

Regulatory reference:	Proprietary method
Claim:	Sebum-regulator and Anti-hair loss
Application field:	Active ingredients or finished products.
Description of the test:	Quantification of the changes in the gene expression of 5α-reductase in murine Balb/3T3 fibroblasts. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II.

Regulatory reference:	Proprietary method
Claim:	Sebum-regulator and Anti-hair loss
Application field:	Active ingredients or finished products.
Description of the test:	Analysis of the in vitro expression of the 5α-reductase enzyme by real time PCR in comparison with untreated substrates for the evaluation of inhibition effects. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II.

Regulatory reference:	Proprietary method
Claim:	Skin whitening and Depigmenting action
Application field:	Active ingredients or finished products.
Description of the test:	Evaluation of the changes in the expression of the tyrosinase gene in human melanoma cells.