Publications

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Soothing effect and Reduce itching
Application field:	Visage and body cosmetics.
Description of the test:	Instrumental measurements with Minolta CR200 colorimeter performed to determine the phototype of the predefined skin region and the correlated MEDu. The hemoglobin content of the target area is measured using a Courage MX18 Mexameter®-Khazaka GmbH. Lenitive, soothing and anti-itching efficacy is measured against histamine, UV or chemically induced irritation.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Reduces skin blemishes, Skin whitening and Depigmenting action
Application field:	Visage and body cosmetic products.
Description of the test:	Instrumental measurements performed using a Minolta CR200 colorimeter will be performed at T=0 and T=X days (to be defined by previous agreement with the sponsor) in order to evaluate changes in the pigmentation of the treated area. The measurements will be performed at each time point in the same area. Acquisition of high definition images will be performed at T=0 and T=X days using a macro objective with color normalization and coaxial circular flash.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Tanning
Application field:	Visage and body cosmetic products.
Description of the test:	A first set of baseline measurements will be performed using a Minolta CR200 Colorimeter in order to identify the phototype of the target skin region and its correlated MEDu. The measurement will be repeated at T=0 and T=X days in order to evaluate changes in skin pigmentation. All measurements will be taken in the same target region. After the treatment, treated and untreated areas will be exposed to 3 irradiation cycles across 5 days using a tanning lamp, in order to quantify the increase in pigmentation. Measurements will be performed using a Colorimeter. High resolution imaging will be performed using a macro objective with coaxial circular flash, after each cycle of simulated sunlight exposure.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Reduces the appearance of cellulite and Toning and firming action
Application field:	Body cosmetic products.
Description of the test:	Morphological analysis of the areas treated with the product by blue laser DermaTOP-V3 body scansion. The instrument can precisely quantify the changes in the volume of panniculus adiposus volumes such as micro and macro-nodules.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Anti-aging, Reduces signs of aging and Illuminating
Application field:	Visage products for mature skin.
Description of the test:	Instrumental evaluation of R0, R2 and R9 parameters. Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity. Manufacturer: Courage-Khazaka GmbH (Germany). The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Firming efficacy, Improves skin tone and smoothness and Improves skin elasticity
Application field:	Visage and body products.
Description of the test:	Measurement of R0 and R2 parameters on 20 healthy volunteers. Instrument: MPA580 Cutometer® for the scientific quantification of the viscoelasticity of the skin. Manufacturer: Courage-Khazaka GmbH (Germany).

Regulatory reference:	Proprietary method
Claim:	Activates the synthesis of collagen, elastin, fibronectin
Application field:	Finished products and active ingredients.
Description of the test:	Real time PCR analysis of the expression of collagen and fibronectin, compared to an untreated control, to determine the efficacy of a product or ingredient in activating the synthesis of these proteins involved in maintaining skin elasticity and prevent aging.

Regulatory reference:	ISO 10993-10
Claim:	Biocompatible
Application field:	Finished products, textiles, solid products by eluate.
Description of the test:	Biocompatibility test: triple analysis for irritation, cytotoxicity and sensitization potential.

Regulatory reference:	Proprietary method
Claim:	Antioxidant
Application field:	Finished products and substances.
Description of the test:	Colorimetric evaluation of the antioxidant activity of the sample in solution on a controlled oxidation reaction. Results in terms of percentage of decrease of oxidation processes caused by free oxygen radicals.

Regulatory reference:	OECD 442D
Claim:	No-skin sensitizer and Hypoallergenic
Application field:	Substances or mixtures.
Description of the test:	The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product.