Publications

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Dermatologically tested
Application field:	Heating and cooling medical devices and cosmetic products.
Description of the test:	Measurement at T=0 before the application and after a preset duration agreed upon with the sponsor; Temperature control using an infrared non-invasive thermometer; Evaluation of the erythemal response through skin colorimetry using a Mexameter® MX18 colorimeter (Courage-Khazaka GmbH – Germany); Evaluation by clinical experts of the general conditions of the skin and epidermal tolerability during use; Subjective evaluation through predefined questionnaire with numerical score system (visual analogical scale) and judgement of the tolerability and heat/chill feeling as perceived during the application of the product; Comparative photography with colorimetric reference captured with a 100 mm macro objective and normalization of the color with coaxial circular flash.

Regulatory reference:	OECD n° 439
Claim:	Non-irritant for the skin
Application field:	Chemical substances or mixtures.
Description of the test:	Evaluation of the irritation potential of chemical substances or mixtures through evaluation of their cytotoxic effect on an in vitro reconstructed model of human skin.

Regulatory reference:	Proprietary method
Claim:	Mucoadhesive effect
Application field:	Finished products for direct application on external mucosae.
Description of the test:	Analysis of the resistance to washing off with and without a basal layer of mucin. Determination of the percentage of resistance against the same product on substrate without mucin.

Regulatory reference:	Proprietary method
Claim:	Protects and repairs the cutaneous barrier
Application field:	Topycal products.
Description of the test:	Measurement of inhibitory efficacy of an active ingredient or finished product against the adhesion of Staphylococcus aureus to epithelial cells.

Regulatory reference:	Proprietary method
Claim:	Inhibition of bacterial adhesion
Application field:	Raw ingredients or finished products.
Description of the test:	Measurement of inhibitory efficacy of an active ingredient or finished product against the adhesion of Staphylococcus aureus to epithelial cells.

Regulatory reference:	ISO 24443:2012
Claim:	Broad spectrum protection (UVA encircled)
Application field:	Sunscreen creams, sticks and cosmetic products.
Description of the test:	Determination of the UVA protection and critical wavelength according to the ISO guideline for broad spectrum protection testing.

Regulatory reference:	Proprietary method
Claim:	Skin whitening and Depigmenting action
Application field:	Active ingredients or finished products.
Description of the test:	Quantification of the inhibition of melanin synthesis in human melanoma cells exposed to the substance or product to be tested, in comparison with untreated controls. The same test can also be used to quantify an increase of melanin synthesis.

Regulatory reference:	Proprietary method
Claim:	Control of the aggregational state of physical filters
Application field:	Sunscreen creams, sticks and cosmetic products.
Description of the test:	Microscopical analysis and high resolution imaging of the aggregation of physical filters. Includes measurement of the size of aggregates, if present.

Regulatory reference:	Proprietary method
Claim:	Evaluation of transcutaneous absorption
Application field:	Solutions or finished products.
Description of the test:	Quantification by HPLC of the amount of an active ingredient permeating the skin barrier of artificial or in vitro 3D skin in Franz’s cells, compared to the total titer of the same substance in the applied product.

Regulatory reference:	Proprietary method
Claim:	Anti-dandruff
Application field:	Anti-dandruff hair products, ingredients or finished products.
Description of the test:	Evaluation of the anti-dandruff efficacy by testing the inhibition of the proliferation of Malassezia furfur by an ingredient or finished product.