Publications

Regulatory reference:	Proprietary method
Claim:	Improves healing process
Application field:	Wound healing products.
Description of the test:	Analysis of the regenerative efficacy on epidermal cell cultures after simulated wounding. Results in terms of percentage of improvement in the regeneration kinetics versus untreated substrate.

Regulatory reference:	Proprietary method
Claim:	Hydrates the skin
Application field:	Complete products.
Description of the test:	Analysis of the expression of aquaporing through real time PCR.

Regulatory reference:	Proprietary method
Claim:	Mucoadhesive effect
Application field:	Finished products for direct application on external mucosae.
Description of the test:	Gravimetric analysis of the adhesion of a product on a layer of mucin against the same product on an untreated plane. Detachment kinetics in terms of percentage of adhesion against a non-adhesive control.

Regulatory reference:	Proprietary method
Claim:	Barrier effect
Application field:	Solutions or finished products.
Description of the test:	Quantification by HPLC of the amount of caffeine permeating the skin barrier of in vitro 3D skin in Franz’s cells, compared to the total titer of the same substance in the applied product.

Regulatory reference:	According to current regulations
Claim:	Titer of substances, Sensitizing metals below risk threshold and Cadmium, Cobalt, Chrome, Nickel free
Description of the test:	Cosmetic products.
Description of the test:	Mineralization/high pressure microwave dissolution and determination through ICP-OES.

Regulatory reference:	Proprietary method based on ISO 24443:2012.
Claim:	Water resistant
Application field:	Sunscreen creams, sticks and cosmetic products.
Description of the test:	Determination of the percentage of water resistance of a sunscreen product on PMMA plates with measurements before and after washes in controlled conditions. The preliminary test requires confirmation through in vivo tests.

Regulatory reference:	Proprietary method
Claim:	Saliva resistant
Application field:	Finished products for oral application.
Description of the test:	Analysis of the resistance to washing off by artificial saliva by gravimetric analysis of the dry weight of the sample before and after washes in controlled conditions.

Regulatory reference:	ISO 16217 and ISO 18861
Claim:	Water resistant or Highly water resistant
Application field:	Sunscreen creams, sticks and cosmetic products.
Description of the test:	Determination of the percentage of persistence of the Sun Protection Factor (SPF) on healthy volunteers after washing in controlled conditions.

Regulatory reference:	Proprietary method
Claim:	Barrier effect
Application field:	Solutions or finished products.
Description of the test:	Quantification by HPLC of the amount of testosterone permeating the skin barrier of in vitro 3D skin in Franz’s cells, compared to the total titer of the same substance in the applied product.

Regulatory reference:	OECD n° 491
Claim:	Non-irritant for the eyes (CLP Labelling)
Application field:	Raw ingredients or finished products.
Description of the test:	Short term exposure analysis by application of each tested substance on SIRC (Statens Seruminstitut Rabbit Cornea) cells for the determination of the effect on cell viability through MTT test.