Behind the Claim: dermatologically tested, hypoallergenic or clinically tested?
Elena Bocchietto1, Stefano Todeschi2
1Director of the testing facility Abich srl, biological and chemical toxicology research laboratory, Verbania (VB), Italy and 2CEO Abich srl, biological and chemical toxicology research laboratory, Verbania (VB), Italy
| Regulatory reference: | OECD 129: on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests; EURL-ECVAM 2011: follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system. |
| Claim: | Biocompatible and Non citotoxic |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | The in vitro LD50 prediction test is designed to test chemicals or mixtures by evaluating their cytotoxic effect on an in vitro model of BALB/c 3T3 murine fibroblasts. |
| Regulatory reference: | Proprietary method |
| Claim: | Depigmenting action |
| Application field: | Active ingredients, mixtures or finished products. |
| Description of the test: | Evaluation of the efficacy of a sample in inhibiting the activity of the tyrosinase enzyme in a controlled in vitro direct reaction. |
| Regulatory reference: | Proprietary method |
| Claim: | Anti-hair loss and Strenghtening |
| Application field: | Active ingredients and finished products. |
| Description of the test: | Quantitative evaluation of the effects of a finished product or active ingredient on cell proliferation and protein synthesis through MTT test and total protein quantification after different periods of exposure. |
Cytotoxicity evaluation of electronic cigarette vapor extract on cultured mammalian fibroblasts (ClearStream-LIFE): comparison with tobacco cigarette smoke extract
Giorgio Romagna1, Elena Allifranchini1, Elena Bocchietto1, Stefano Todeschi1, Mara Esposito1, and Konstantinos E. Farsalinos2
1Abich srl, biological and chemical toxicology research laboratory, Verbania (VB), Italy and 2Department of Cardiology, Onassis Cardiac Surgery Cente, Kallithea, Greece
Comparison of the Cytotoxic Potential of Cigarette Smoke and Electronic Cigarette Vapour Extract on Cultured Myocardial Cells
Konstantinos E. Farsalinos 1,*, Giorgio Romagna 2, Elena Allifranchini 2, Emiliano Ripamonti 2, Elena Bocchietto 2, Stefano Todeschi 2, Dimitris Tsiapras 1, Stamatis Kyrzopoulos 1 and Vassilis Voudris 1
1 Onassis Cardiac Surgery Center, Sygrou 356, Kallithea 17674, Greece;
2 ABICH S.r.l, Biological and Chemical Toxicology Research Laboratory, Via 42 Martiri,213/B-28924 Verbania (VB), Italy;
Decontamination of tetramethylammonium hydroxide (TMAH) splashes: promising results with Diphoterine® in vitro
Céline Fosse1, Laurence Mathieu1, Alan H. Hall2,3, Elena Bocchietto4, François Burgher1, Michel Fischbach4, and Howard I. Maibach5
1Laboratoire Prevor, Valmondois, France, 2Toxicology Consulting and Medical Translating Services, Inc. (TCMTS, Inc.), Laramie, Wyoming, USA, 3Colorado School of Public Health, Denver, Colorado, USA, 4ABICH Srl, Verbano, Italy, and 5Department of Dermatology, University of California–San Francisco, San Francisco, California, USA
HUMAN MONOCYTOID THP-1 CELL LINE VERSUS MONOCYTE-DERIVED HUMAN IMMATURE DENDRITIC CELLS AS IN VITRO MODELS FOR PREDICTING THE SENSITISING POTENTIAL OF CHEMICALS
E. BOCCHIETTO, C. PAOLUCCI1, D. BREDA1, E. SABBIONI2 and S.E. BURASTERO1
Abich, Tecnoparco, Lago Maggiore, Verbania; 1San Raffaele Scientific Institute, Department of Biotechnology, Milan; 2European Centre for the Validation of Alternative Methods, Joint Research Centre, Ispra, Varese, Italy
| Regulatory reference: | OECD n° 492 |
| Claim: | Non-irritant for the eyes |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action. |
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Ophthalmologically tested |
| Application field: | Eye contour cosmetics or products that require to confirm the compatibility with the periocular mucosa in normal usage conditions. |
| Description of the test: | An ophthalmology specialist will inspect the target area, and especially the eyelids, before the application using a fissure lamp, in order to confirm the absence of swelling, hyperemia, dryness or desquamation. The inspection also includes any alterations of the eyelashes. In the eye itself, the integrity of the cornea and conjunctiva, the absence of hyperemia, and signs of conjunctival or tarsal irritation are verified. Finally, the absence of qualitative and quantitative alterations in the tear film. The specialist then evaluates and scores erythemal response, tearing, edema and dryness after the product has been used for 30 minutes, 2 days and 30 days to quantify possible alterations induced by the sample. The volunteers also provide subjective evaluations of itching, burning, stinging or foreign body response feeling, and/or sight clouding, at the same endpoints, using the VAS (Visual Analogical Scale) score system. |
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